Mesenchymal Stromal Cells Derived Extracellular Vesicles Ameliorate Acute Renal Ischemia Reperfusion Injury by Inhibition of Mitochondrial Fission through miR-30

Mesenchymal Stromal Cells Derived Extracellular Vesicles Ameliorate Acute Renal Ischemia Reperfusion Injury by Inhibition of Mitochondrial Fission through miR-30

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Background.The immoderation of mitochondrial fission is one of the main contributors in ischemia reperfusion injury (IRI) and mesenchymal stromal cells (MSCs) derived extracellular vesicles have been 2 lb m&m bag regarded as a potential therapy method.Here, we hypothesized that extracellular vesicles (EVs) derived from human Wharton Jelly mesenchymal stromal cells (hWJMSCs) ameliorate acute renal IRI by inhibiting mitochondrial fission through miR-30b/c/d.

Methods.EVs isolated from the condition medium of MCS were injected intravenously in rats immediately after monolateral nephrectomy and renal pedicle occlusion for 45 minutes.Animals were sacrificed at 24 h after reperfusion and samples were collected.

MitoTracker Red staining was used to see the morphology of the mitochondria.The expression of DRP1 was measured by western blot.miR-30 in EVs and rat tubular epithelial cells was assessed by qRT-PCR.

Apoptosis pathway was identified by immunostaining.Results.We found that the expression of miR-30 in injured kidney tissues was declined and mitochondrial dynamics turned to fission.

But they were both restored in EVs group in parallel with reduced cell apoptosis.What is more, when the miR-30 antagomirs were used to reduce the miRNA levels, all the related effects of EVs reduced remarkably.Conclusion.

A single administration of hWJMSC-EVs could protect the kidney from IRI by inhibition of mitochondrial fission maxbotix hrlv ez1 via miR-30.